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Human Protein Atlas nos2 expression
<t> NOS2 </t> transcripts in the ENSEMBL database

Nos2 Expression, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nos2 expression/product/Human Protein Atlas
Average 90 stars, based on 1 article reviews

nos2 expression - by Bioz Stars, 2026-03

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1) Product Images from "A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types"

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

Journal: Cell Communication and Signaling : CCS

doi: 10.1186/s12964-022-00855-x

Figure Legend Snippet: NOS2 transcripts in the ENSEMBL database

Techniques Used:

NOS2 mRNA isoform expression in the human sigmoid colon and small intestine. RNA-Seq data (gene expression omnibus GSM1010942, GSM1010940) were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3). There was no expression of NOS2-2 and -3

Figure Legend Snippet: NOS2 mRNA isoform expression in the human sigmoid colon and small intestine. RNA-Seq data (gene expression omnibus GSM1010942, GSM1010940) were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3). There was no expression of NOS2-2 and -3

Techniques Used: Expressing, RNA Sequencing, Gene Expression

NOS2 mRNA isoform expression in isolated human islets treated with or without a cytokine mixture. RNA-Seq data (Bioproject PRJNA151601 ) were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3) expressed in isolated human islets incubated with (CM) or without a cytokine mixture (co) containing IL-1β and IFN-γ. ***FDR p value < 0.001, ns FDR p value > 0.05 versus co

Figure Legend Snippet: NOS2 mRNA isoform expression in isolated human islets treated with or without a cytokine mixture. RNA-Seq data (Bioproject PRJNA151601 ) were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3) expressed in isolated human islets incubated with (CM) or without a cytokine mixture (co) containing IL-1β and IFN-γ. ***FDR p value < 0.001, ns FDR p value > 0.05 versus co

Techniques Used: Expressing, Isolation, RNA Sequencing, Incubation

NOS2 mRNA isoform expression in different placenta-derived trophoblast stem cells (CT29, CT30) and H1- or H9-ESC induced to differentiate to trophoblast stem cells. RNA-Seq data (Bioproject PRJNA565033 were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of NOS2 mRNA isoforms at different differentiation stages. ***FDR p value < 0.001, ns FDR-value > 0.05 versus untreated H9-ESC

Figure Legend Snippet: NOS2 mRNA isoform expression in different placenta-derived trophoblast stem cells (CT29, CT30) and H1- or H9-ESC induced to differentiate to trophoblast stem cells. RNA-Seq data (Bioproject PRJNA565033 were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of NOS2 mRNA isoforms at different differentiation stages. ***FDR p value < 0.001, ns FDR-value > 0.05 versus untreated H9-ESC

Techniques Used: Expressing, Derivative Assay, RNA Sequencing

NOS2 mRNA isoform expression in human iPSC from normal (WT) and down syndrome (DOWN) donors induced to differentiate to neurons . RNA-Seq data (Bioproject PRJDB1099 ; FANTOM5) were analyzed for human NOS2 mRNA expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3) at different time points (day 0 to day 18). ***FDR p value < 0.001, *FDR p value < 0.05, ns FDR p value > 0.05 versus day 0

Figure Legend Snippet: NOS2 mRNA isoform expression in human iPSC from normal (WT) and down syndrome (DOWN) donors induced to differentiate to neurons . RNA-Seq data (Bioproject PRJDB1099 ; FANTOM5) were analyzed for human NOS2 mRNA expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3) at different time points (day 0 to day 18). ***FDR p value < 0.001, *FDR p value < 0.05, ns FDR p value > 0.05 versus day 0

Techniques Used: Expressing, RNA Sequencing

NOS2 mRNA- and protein expression in four different human iPSC lines induced to differentiate to neurons. Four different human iPSCs lines were generated from PBMC of three different donors . RNA and protein were isolated from these 4 different human iPSC lines induced to differentiate to neurons at different time points (day 0 to 60). A NOS2-mRNA and 18S rRNA expression were analyzed using the qRT-PCR method. NOS2 mRNA expression was normalized to the 18S rRNA expression. The relative NOS2 mRNA expression in the cells treated for 18 days was set to 100%. Shown is the summary of the analysis of the four different iPSC cell lines. The values represent the mean ± SEM of n = 12 different isolated RNAs at each time point. (*** p < 0.001, * p < 0.05, ns not significant vs. iPSC treated for zero days; 1-way Anova with Dunnett's multiple comparisons test). B NOS2- and GAPDH protein expression in DLD1 cells (DLD1) incubated with (CM) or without (Co) a cytokine mixture to induce the human iNOS expression and ILB-C89bf (ILB-C89bf 18 days) cells stimulated to differentiate to neurons for 18 days were analyzed using the western blot method. Shown are the detected bands of NOS2- and GAPDH protein (analyzed using either the anti-NOS2- or the anti-GAPDH antibody)

Figure Legend Snippet: NOS2 mRNA- and protein expression in four different human iPSC lines induced to differentiate to neurons. Four different human iPSCs lines were generated from PBMC of three different donors . RNA and protein were isolated from these 4 different human iPSC lines induced to differentiate to neurons at different time points (day 0 to 60). A NOS2-mRNA and 18S rRNA expression were analyzed using the qRT-PCR method. NOS2 mRNA expression was normalized to the 18S rRNA expression. The relative NOS2 mRNA expression in the cells treated for 18 days was set to 100%. Shown is the summary of the analysis of the four different iPSC cell lines. The values represent the mean ± SEM of n = 12 different isolated RNAs at each time point. (*** p < 0.001, * p < 0.05, ns not significant vs. iPSC treated for zero days; 1-way Anova with Dunnett's multiple comparisons test). B NOS2- and GAPDH protein expression in DLD1 cells (DLD1) incubated with (CM) or without (Co) a cytokine mixture to induce the human iNOS expression and ILB-C89bf (ILB-C89bf 18 days) cells stimulated to differentiate to neurons for 18 days were analyzed using the western blot method. Shown are the detected bands of NOS2- and GAPDH protein (analyzed using either the anti-NOS2- or the anti-GAPDH antibody)

Techniques Used: Expressing, Generated, Isolation, Quantitative RT-PCR, Incubation, Western Blot

NOS2 mRNA isoform analysis using 5′RACE and isoform specific qRT-PCR methods. A Schematic summary of the results of 5′-RACE analyses using RNA isolated at day 18 from human iPSC induced to differentiate to neurons. RNA was isolated from 4 different human iPSC lines (samples C16-1, C16-2, C89-2, C89-3) induced to differentiate to neurons for 18 days. As control RNA was isolated from human DLD1 cells (DLD1-2 and DLD1-2) treated with a cytokine mixture for 6 h to induce NOS2 mRNA expression. Then 5′-RACE experiments were performed (as described in “Methods” section) using the indicated oligonucleotides as 3′-primer (5′RACE-rev = primer used for the RT-reaction; 3P2/3P3 primers used for the PCR-reaction). The final PCR products were isolated, cloned into pCR-Script and sequenced. The alignment of these sequences to the human NOS2-1 and -2 mRNA are indicated as filled arrows. The sequences of the fragments are shown in Additional file : Fig. S10. B/C NOS2-mRNA-isoform expression analysis using isoform specific qRT-PCR experiments. RNA was isolated at different time points from 2 different human iPSC lines (iLB-C16bm s6 iPS andiLB-C89bf s4 iPCs) induced to differentiate to neurons for 60 days. The RT reaction was performed with the RT-rev primer (see A ). Taqman qPCR reactions were performed with mRNA isoform specific primer pairs and taqman probes (NOS2-1: NOS2-1_P, NOS2-1_3P and NOS2-1_Taq; NOS2-2: NOS2-2_5P, NOS2-2_3P and NOS2-2_Taq—see A ). For normalization also the GAPDH mRNA expression was measured. B The normalized NOS2-1 mRNA expression values were related to the CM induced NOS2-1 mRNA expression in DLD-1 cells (CM = 100%). Also, the expression level of the NOS2-1 mRNA in untreated (co) DLD-1 cells were determined. (*** p < 0.001, ns not significant vs. iPSC treated for 18 days; 1-way Anova with Dunnett’s multiple comparisons test). C The normalized NOS2-2 mRNA expression values were related to the NOS2-2 mRNA expression in iPSC treated for 18 days (day 18 = 100%). (** p < 0.05, vs. iPSC treated for 18 days; 1-way Anova with Dunnett’s multiple comparisons test)

Figure Legend Snippet: NOS2 mRNA isoform analysis using 5′RACE and isoform specific qRT-PCR methods. A Schematic summary of the results of 5′-RACE analyses using RNA isolated at day 18 from human iPSC induced to differentiate to neurons. RNA was isolated from 4 different human iPSC lines (samples C16-1, C16-2, C89-2, C89-3) induced to differentiate to neurons for 18 days. As control RNA was isolated from human DLD1 cells (DLD1-2 and DLD1-2) treated with a cytokine mixture for 6 h to induce NOS2 mRNA expression. Then 5′-RACE experiments were performed (as described in “Methods” section) using the indicated oligonucleotides as 3′-primer (5′RACE-rev = primer used for the RT-reaction; 3P2/3P3 primers used for the PCR-reaction). The final PCR products were isolated, cloned into pCR-Script and sequenced. The alignment of these sequences to the human NOS2-1 and -2 mRNA are indicated as filled arrows. The sequences of the fragments are shown in Additional file : Fig. S10. B/C NOS2-mRNA-isoform expression analysis using isoform specific qRT-PCR experiments. RNA was isolated at different time points from 2 different human iPSC lines (iLB-C16bm s6 iPS andiLB-C89bf s4 iPCs) induced to differentiate to neurons for 60 days. The RT reaction was performed with the RT-rev primer (see A ). Taqman qPCR reactions were performed with mRNA isoform specific primer pairs and taqman probes (NOS2-1: NOS2-1_P, NOS2-1_3P and NOS2-1_Taq; NOS2-2: NOS2-2_5P, NOS2-2_3P and NOS2-2_Taq—see A ). For normalization also the GAPDH mRNA expression was measured. B The normalized NOS2-1 mRNA expression values were related to the CM induced NOS2-1 mRNA expression in DLD-1 cells (CM = 100%). Also, the expression level of the NOS2-1 mRNA in untreated (co) DLD-1 cells were determined. (*** p < 0.001, ns not significant vs. iPSC treated for 18 days; 1-way Anova with Dunnett’s multiple comparisons test). C The normalized NOS2-2 mRNA expression values were related to the NOS2-2 mRNA expression in iPSC treated for 18 days (day 18 = 100%). (** p < 0.05, vs. iPSC treated for 18 days; 1-way Anova with Dunnett’s multiple comparisons test)

Techniques Used: Quantitative RT-PCR, Isolation, Control, Expressing, Clone Assay

The NOS2-2 protein is functional. DLD-1_TR7 cells stably expressing a tetracycline repressor were transiently transfected with pcDNA4/TO_NOS2-1_cds_3UTR or pcDNA4/TO_NOS2-2_cds_3UTR encoding the NOS2-1 or -2 protein. To normalize the transfection efficiency, pRL-EF1α (encoding for a renilla luciferase) was cotransfected as well. After transfection, the cells were incubated with (Tet) or without (co) 500 ng/ml tetracycline for 24 h. Then the supernatants of the cells were used for nitrate concentration determination by the Griess assay. The cells were lyzed and renilla luciferase activity was measured. The nitrate concentrations determined were normalized to the renilla luciferase data. Shown is the summary of the analysis of the four different transfection experiments. The values represent the mean ± SEM of n = 8 different wells, (*** p < 0.001; * p < 0.05, ns not significant;1-way Anova with Dunnett's multiple comparisons test)

Figure Legend Snippet: The NOS2-2 protein is functional. DLD-1_TR7 cells stably expressing a tetracycline repressor were transiently transfected with pcDNA4/TO_NOS2-1_cds_3UTR or pcDNA4/TO_NOS2-2_cds_3UTR encoding the NOS2-1 or -2 protein. To normalize the transfection efficiency, pRL-EF1α (encoding for a renilla luciferase) was cotransfected as well. After transfection, the cells were incubated with (Tet) or without (co) 500 ng/ml tetracycline for 24 h. Then the supernatants of the cells were used for nitrate concentration determination by the Griess assay. The cells were lyzed and renilla luciferase activity was measured. The nitrate concentrations determined were normalized to the renilla luciferase data. Shown is the summary of the analysis of the four different transfection experiments. The values represent the mean ± SEM of n = 8 different wells, (*** p < 0.001; * p < 0.05, ns not significant;1-way Anova with Dunnett's multiple comparisons test)

Techniques Used: Functional Assay, Stable Transfection, Expressing, Transfection, Luciferase, Incubation, Concentration Assay, Griess Assay, Activity Assay

Figure Legend Snippet:

Techniques Used:

Image Search Results

Ets-1 transcriptional activity in response to NOS2 expression and NO signaling . (a) Western blot of NOS2 and Ets-1 (thr 38) phosphorylation in MDA-MB-468 cells transfected with control plasmid and NOS2 expression plasmid in the presence of NOS2 substrate (L-Arg) or inhibitor (AG). (b) Western blot of phospho-Ets-1 (thr 38) compared to total Ets-1 in serum-starved cells exposed to either EGF (10 ng/ml) or DETANO. (c) Ets-luciferase activity in MDA-MB-468 cells transfected with either control or NOS2 expression plasmid and cultured in the presence of L-Arg or AG. Data represent mean fold luciferase activity compared to control plasmid incubated with L-Arg. (d) Ets-luciferase activity in serum-starved cells treated with either EGF or DETANO. Data represent mean fold luciferase activity compared to untreated control. Significant luciferase activity (** P < 0.01) was determined by one-way ANOVA from at least three independent experiments. AG, aminoguanidine; ANOVA, analysis of variance; DETANO, diethlylenetriamine NONOate; EGF, epidermal growth factor; Ets-1, erythroblastosis virus E26 oncogene homolog 1; L-Arg, L-arginine; NOS2, nitric oxide synthase.

Journal: Breast Cancer Research : BCR

Article Title: Ets-1 is a transcriptional mediator of oncogenic nitric oxide signaling in estrogen receptor-negative breast cancer

doi: 10.1186/bcr3319

Figure Lengend Snippet: Ets-1 transcriptional activity in response to NOS2 expression and NO signaling . (a) Western blot of NOS2 and Ets-1 (thr 38) phosphorylation in MDA-MB-468 cells transfected with control plasmid and NOS2 expression plasmid in the presence of NOS2 substrate (L-Arg) or inhibitor (AG). (b) Western blot of phospho-Ets-1 (thr 38) compared to total Ets-1 in serum-starved cells exposed to either EGF (10 ng/ml) or DETANO. (c) Ets-luciferase activity in MDA-MB-468 cells transfected with either control or NOS2 expression plasmid and cultured in the presence of L-Arg or AG. Data represent mean fold luciferase activity compared to control plasmid incubated with L-Arg. (d) Ets-luciferase activity in serum-starved cells treated with either EGF or DETANO. Data represent mean fold luciferase activity compared to untreated control. Significant luciferase activity (** P < 0.01) was determined by one-way ANOVA from at least three independent experiments. AG, aminoguanidine; ANOVA, analysis of variance; DETANO, diethlylenetriamine NONOate; EGF, epidermal growth factor; Ets-1, erythroblastosis virus E26 oncogene homolog 1; L-Arg, L-arginine; NOS2, nitric oxide synthase.

Article Snippet: Cells were transfected with 4 µg pCMV6-XL4 (empty vector) or pCMV6-XL4-human NOS2 (NM_000625) (OriGene Technologies, Rockville, MD, USA) by electroporation using the Amaxa Nucleofector kit V (Lonza, Walkersville, MD, USA) and then grown for 48 hours under normal conditions before further treatment or analysis.

Techniques: Activity Assay, Expressing, Western Blot, Transfection, Plasmid Preparation, Luciferase, Cell Culture, Incubation

NO activation of Ets-1 requires the MEK/ERK signaling pathway . (a) Western blot of relative MEK1/2 (ser 217/221) and ERK1/2 (thr 202/tyr204) phosphorylation in MDA-MB-468 cells transfected with control or NOS2 expression plasmid and cultured with L-Arg or AG. (b) Western blot of relative MEK1/2 (ser 217/221) and ERK1/2 (thr 202/tyr204) phosphorylation in serum-starved cells exposed to EGF or DETANO. (c) Western blot of ERK1/2 (thr 202/tyr204) and Ets-1 (thr 38) phosphorylation in serum-starved MDA-MB-468 cells exposed to EGF (10 ng/ml) or DETANO (0.5 mM), with and without the MEK inhibitor PD 184161. (d) Ets-luciferase activity in serum-starved MDA-MB-468 cells exposed to conditions described in (c). AG, aminoguanidine; DETANO, diethlylenetriamine NONOate; EGF, epidermal growth factor; ERK, extracellular signal-regulated protein kinase; Ets-1, erythroblastosis virus E26 oncogene homolog 1; L-Arg, L-arginine; MEK, mitogen-activated protein kinase; NOS2, nitric oxide synthase.

Journal: Breast Cancer Research : BCR

Article Title: Ets-1 is a transcriptional mediator of oncogenic nitric oxide signaling in estrogen receptor-negative breast cancer

doi: 10.1186/bcr3319

Figure Lengend Snippet: NO activation of Ets-1 requires the MEK/ERK signaling pathway . (a) Western blot of relative MEK1/2 (ser 217/221) and ERK1/2 (thr 202/tyr204) phosphorylation in MDA-MB-468 cells transfected with control or NOS2 expression plasmid and cultured with L-Arg or AG. (b) Western blot of relative MEK1/2 (ser 217/221) and ERK1/2 (thr 202/tyr204) phosphorylation in serum-starved cells exposed to EGF or DETANO. (c) Western blot of ERK1/2 (thr 202/tyr204) and Ets-1 (thr 38) phosphorylation in serum-starved MDA-MB-468 cells exposed to EGF (10 ng/ml) or DETANO (0.5 mM), with and without the MEK inhibitor PD 184161. (d) Ets-luciferase activity in serum-starved MDA-MB-468 cells exposed to conditions described in (c). AG, aminoguanidine; DETANO, diethlylenetriamine NONOate; EGF, epidermal growth factor; ERK, extracellular signal-regulated protein kinase; Ets-1, erythroblastosis virus E26 oncogene homolog 1; L-Arg, L-arginine; MEK, mitogen-activated protein kinase; NOS2, nitric oxide synthase.

Techniques: Activation Assay, Western Blot, Transfection, Expressing, Plasmid Preparation, Cell Culture, Luciferase, Activity Assay

Ets-1 activation by NO requires Ras signaling . Western blot of S-nitrosylated, active and total Ras in serum-starved MDA-MB-468 cells (a) transfected with NOS2 expression plasmid and treated with L-Arg or AG and (b) treated with EGF or DETANO. (c) Western blot of Ras-SNO and total Ras from MDA-MB-468 cells exposed to DETANO (0.5 mM) alone or in combination with N-acetyl cysteine (NAC) or sodium azide. (d) Ets-luciferase activity in MDA-MB-468 cells exposed to conditions as above. Significance to DETANO was determined by one-way ANOVA (** P < 0.01). (e) Western blot of Ras and Ets-1 (thr 38) phosphorylation in serum-starved MDA-MB-468 cells exposed to EGF or DETANO (0.5 mM) in the presence or absence of FTS. (f) Western blot of Ets-1 (thr 38) phosphorylation in serum-starved MDA-MB-468 cells exposed to EGF or DETANO (0.5 mM) in the presence or absence of Gö 6976. (g) Ets-luciferase activity in serum-starved MDA-MB-468 cells exposed to EGF or DETANO (0.5 mM) in the presence or absence of FTS or Gö 6976. Significance compared to control was determined by one-way ANOVA (* P < 0.05). (h) Schematic representing the NO-sensitive Ras/MEK/ERK/Ets-1 signaling pathway. AG, aminoguanidine; ANOVA, analysis of variance; DETANO, diethlylenetriamine NONOate; EGF, epidermal growth factor; ERK, extracellular signal-regulated protein kinase; Ets-1, erythroblastosis virus E26 oncogene homolog 1; FTS, farnesylthiosalicylic acid; L-Arg, L-arginine; MEK, mitogen-activated protein kinase; NOS2, nitric oxide synthase.

Journal: Breast Cancer Research : BCR

Article Title: Ets-1 is a transcriptional mediator of oncogenic nitric oxide signaling in estrogen receptor-negative breast cancer

doi: 10.1186/bcr3319

Figure Lengend Snippet: Ets-1 activation by NO requires Ras signaling . Western blot of S-nitrosylated, active and total Ras in serum-starved MDA-MB-468 cells (a) transfected with NOS2 expression plasmid and treated with L-Arg or AG and (b) treated with EGF or DETANO. (c) Western blot of Ras-SNO and total Ras from MDA-MB-468 cells exposed to DETANO (0.5 mM) alone or in combination with N-acetyl cysteine (NAC) or sodium azide. (d) Ets-luciferase activity in MDA-MB-468 cells exposed to conditions as above. Significance to DETANO was determined by one-way ANOVA (** P < 0.01). (e) Western blot of Ras and Ets-1 (thr 38) phosphorylation in serum-starved MDA-MB-468 cells exposed to EGF or DETANO (0.5 mM) in the presence or absence of FTS. (f) Western blot of Ets-1 (thr 38) phosphorylation in serum-starved MDA-MB-468 cells exposed to EGF or DETANO (0.5 mM) in the presence or absence of Gö 6976. (g) Ets-luciferase activity in serum-starved MDA-MB-468 cells exposed to EGF or DETANO (0.5 mM) in the presence or absence of FTS or Gö 6976. Significance compared to control was determined by one-way ANOVA (* P < 0.05). (h) Schematic representing the NO-sensitive Ras/MEK/ERK/Ets-1 signaling pathway. AG, aminoguanidine; ANOVA, analysis of variance; DETANO, diethlylenetriamine NONOate; EGF, epidermal growth factor; ERK, extracellular signal-regulated protein kinase; Ets-1, erythroblastosis virus E26 oncogene homolog 1; FTS, farnesylthiosalicylic acid; L-Arg, L-arginine; MEK, mitogen-activated protein kinase; NOS2, nitric oxide synthase.

Techniques: Activation Assay, Western Blot, Transfection, Expressing, Plasmid Preparation, Luciferase, Activity Assay

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 transcripts in the ENSEMBL database

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques:

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 mRNA isoform expression in the human sigmoid colon and small intestine. RNA-Seq data (gene expression omnibus GSM1010942, GSM1010940) were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3). There was no expression of NOS2-2 and -3

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Expressing, RNA Sequencing, Gene Expression

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 mRNA isoform expression in isolated human islets treated with or without a cytokine mixture. RNA-Seq data (Bioproject PRJNA151601 ) were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3) expressed in isolated human islets incubated with (CM) or without a cytokine mixture (co) containing IL-1β and IFN-γ. ***FDR p value < 0.001, ns FDR p value > 0.05 versus co

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Expressing, Isolation, RNA Sequencing, Incubation

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 mRNA isoform expression in different placenta-derived trophoblast stem cells (CT29, CT30) and H1- or H9-ESC induced to differentiate to trophoblast stem cells. RNA-Seq data (Bioproject PRJNA565033 were analyzed for human NOS2 mRNA isoform expression. Shown are the mean ± SEM of the rpkm values of NOS2 mRNA isoforms at different differentiation stages. ***FDR p value < 0.001, ns FDR-value > 0.05 versus untreated H9-ESC

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Expressing, Derivative Assay, RNA Sequencing

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 mRNA isoform expression in human iPSC from normal (WT) and down syndrome (DOWN) donors induced to differentiate to neurons . RNA-Seq data (Bioproject PRJDB1099 ; FANTOM5) were analyzed for human NOS2 mRNA expression. Shown are the mean ± SEM of the rpkm values of the different NOS2 mRNA isoforms (NOS2-1, -2 and -3) at different time points (day 0 to day 18). ***FDR p value < 0.001, *FDR p value < 0.05, ns FDR p value > 0.05 versus day 0

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Expressing, RNA Sequencing

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 mRNA- and protein expression in four different human iPSC lines induced to differentiate to neurons. Four different human iPSCs lines were generated from PBMC of three different donors . RNA and protein were isolated from these 4 different human iPSC lines induced to differentiate to neurons at different time points (day 0 to 60). A NOS2-mRNA and 18S rRNA expression were analyzed using the qRT-PCR method. NOS2 mRNA expression was normalized to the 18S rRNA expression. The relative NOS2 mRNA expression in the cells treated for 18 days was set to 100%. Shown is the summary of the analysis of the four different iPSC cell lines. The values represent the mean ± SEM of n = 12 different isolated RNAs at each time point. (*** p < 0.001, * p < 0.05, ns not significant vs. iPSC treated for zero days; 1-way Anova with Dunnett's multiple comparisons test). B NOS2- and GAPDH protein expression in DLD1 cells (DLD1) incubated with (CM) or without (Co) a cytokine mixture to induce the human iNOS expression and ILB-C89bf (ILB-C89bf 18 days) cells stimulated to differentiate to neurons for 18 days were analyzed using the western blot method. Shown are the detected bands of NOS2- and GAPDH protein (analyzed using either the anti-NOS2- or the anti-GAPDH antibody)

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Expressing, Generated, Isolation, Quantitative RT-PCR, Incubation, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: NOS2 mRNA isoform analysis using 5′RACE and isoform specific qRT-PCR methods. A Schematic summary of the results of 5′-RACE analyses using RNA isolated at day 18 from human iPSC induced to differentiate to neurons. RNA was isolated from 4 different human iPSC lines (samples C16-1, C16-2, C89-2, C89-3) induced to differentiate to neurons for 18 days. As control RNA was isolated from human DLD1 cells (DLD1-2 and DLD1-2) treated with a cytokine mixture for 6 h to induce NOS2 mRNA expression. Then 5′-RACE experiments were performed (as described in “Methods” section) using the indicated oligonucleotides as 3′-primer (5′RACE-rev = primer used for the RT-reaction; 3P2/3P3 primers used for the PCR-reaction). The final PCR products were isolated, cloned into pCR-Script and sequenced. The alignment of these sequences to the human NOS2-1 and -2 mRNA are indicated as filled arrows. The sequences of the fragments are shown in Additional file : Fig. S10. B/C NOS2-mRNA-isoform expression analysis using isoform specific qRT-PCR experiments. RNA was isolated at different time points from 2 different human iPSC lines (iLB-C16bm s6 iPS andiLB-C89bf s4 iPCs) induced to differentiate to neurons for 60 days. The RT reaction was performed with the RT-rev primer (see A ). Taqman qPCR reactions were performed with mRNA isoform specific primer pairs and taqman probes (NOS2-1: NOS2-1_P, NOS2-1_3P and NOS2-1_Taq; NOS2-2: NOS2-2_5P, NOS2-2_3P and NOS2-2_Taq—see A ). For normalization also the GAPDH mRNA expression was measured. B The normalized NOS2-1 mRNA expression values were related to the CM induced NOS2-1 mRNA expression in DLD-1 cells (CM = 100%). Also, the expression level of the NOS2-1 mRNA in untreated (co) DLD-1 cells were determined. (*** p < 0.001, ns not significant vs. iPSC treated for 18 days; 1-way Anova with Dunnett’s multiple comparisons test). C The normalized NOS2-2 mRNA expression values were related to the NOS2-2 mRNA expression in iPSC treated for 18 days (day 18 = 100%). (** p < 0.05, vs. iPSC treated for 18 days; 1-way Anova with Dunnett’s multiple comparisons test)

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Quantitative RT-PCR, Isolation, Control, Expressing, Clone Assay

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet: The NOS2-2 protein is functional. DLD-1_TR7 cells stably expressing a tetracycline repressor were transiently transfected with pcDNA4/TO_NOS2-1_cds_3UTR or pcDNA4/TO_NOS2-2_cds_3UTR encoding the NOS2-1 or -2 protein. To normalize the transfection efficiency, pRL-EF1α (encoding for a renilla luciferase) was cotransfected as well. After transfection, the cells were incubated with (Tet) or without (co) 500 ng/ml tetracycline for 24 h. Then the supernatants of the cells were used for nitrate concentration determination by the Griess assay. The cells were lyzed and renilla luciferase activity was measured. The nitrate concentrations determined were normalized to the renilla luciferase data. Shown is the summary of the analysis of the four different transfection experiments. The values represent the mean ± SEM of n = 8 different wells, (*** p < 0.001; * p < 0.05, ns not significant;1-way Anova with Dunnett's multiple comparisons test)

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques: Functional Assay, Stable Transfection, Expressing, Transfection, Luciferase, Incubation, Concentration Assay, Griess Assay, Activity Assay

Journal: Cell Communication and Signaling : CCS

Article Title: A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types

doi: 10.1186/s12964-022-00855-x

Figure Lengend Snippet:

Article Snippet: In addition to NOS2 expression in the different colon parts, the human protein atlas describes NOS2 expression in human placental cells.

Techniques:

Journal: iScience

Article Title: STING regulates peripheral nerve regeneration and colony stimulating factor 1 receptor (CSF1R) processing in microglia

doi: 10.1016/j.isci.2021.103434

Figure Lengend Snippet:

Article Snippet: Nos2 - Taqman Gene Expression Assay , Thermo Fisher Scientific , Mm00440502_m1.

Techniques: Recombinant, Comparison, Gene Expression, Software, Cell Analysis

Journal: Immunity

Article Title: The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair

doi: 10.1016/j.immuni.2019.06.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Nos2 gene expression assay (mouse) , Thermo Fisher Scientific , Mm00440502_m1.

Techniques: Recombinant, Staining, Cytokine Assay, Enzyme-linked Immunosorbent Assay, Uric Acid Assay, Gene Expression, Software

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